METHODOLOGY
MODEL SPECIES, METHODS AND VALIDATION
DARWIN proposes to deliver validated analytical and digital tools for screening, detection, identification, and quantification of NGT products in the food chain under an interdisciplinary and co-creative approach.
2 PLANT SPECIES: RICE AND TOMATO
DARWIN’s analytical detection is based on the model species of rice and tomato.
The genotypes portfolio of DARWIN is intended to include different lines of rice and tomato.
Why rice and tomato?
- They have contrasting characteristics
- They are food samples all over the world
METHODS:
- ENHANCED RT-qPCR (LNA, DPO, HiDi)
- ENHANCED RT-qPCR (RINA- se H)
- TRIPLEX RT- qPCR (LNA, DPO, HiDi)
- DUPLEX DROPLET dPCR
- PENTAPLEX NANOPLATE dPCR
- PCR-ENRICHED MULTI TARGET SEQUENCING
- WHOLE GENOME SEQUENCING (WGS)
- METAGENOMIC HIGH-THROUGHPUT SEQUENCING
3-LEVEL SCHEME FOR 
VALIDATION OF TECHNOLOGIES
- Intra-lab validation
- Method transferability validation
- Full Ring Trial
